You can ensure your sample is representative by resuspending the solution by pipetting up and down a few times before taking your sample. Start by taking a representative sample of your cell population. The various grids allow you to count cells of different sizes.īefore you begin cell counting with your hemocytometer, you need to do some preparation. As shown in the diagram, each square is further divided into 16, 100, or 400 smaller squares. The 3×3 mm counting grid on the Improved Neubauer is subdivided into nine 1×1mm squares. Grids are engraved onto the surface to make cell counting easier and more precise. The Improved Neubauer has an H-shaped indent at the center of the slide that separates the space into two counting chambers. The most commonly used is the ‘Improved Neubauer’ chamber. There are several types of hemocytometer, all with different counting grids. A hemocytometer is a specialized slide used for cell counting with a microscope.
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